Detection of other special sample please contact our technical support. Creative diagnostics provides homocysteine antibodies and elisa kits for science research use, feel free to contact us. Kt60099 intended use this hcy elisa kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures. Hcy elisa kit human hcy homocysteine elisa kit mybiosource. Arg80875 human homocysteine hcy elisa kit is an enzyme immunoassay kit for the quantification of human homocysteine hcy in serum and plasma edta.
The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine selective enzyme. For the quantitative determination of endogenic human homocysteine hcy concentrations in. Principle of the assay this experiment use doublesandwich elisa technique and the elisa kit provided is typical. Covid 19 xpressbio is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Test principle the microtiter plate provided in human homocysteine elisa kit has been precoated with an antibody specific to homocysteine. Mouse homocysteine hcy elisa kit mybiosource mbs260152. Lsf36528 it is important that you read this entire manual carefully before starting your experiment.
Homocysteine inducible, endoplasmic reticulum stressinducible, ubiquitinlike domain member 1 elisa kits homogentisate 1,2dioxygenase elisa kits homolog of yeast recqmediated genome instability 1 elisa kits. Maxdiscovery homocysteine enzymatic assay kit bioo. The precoated antibody is mouse hcy monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Homocysteine elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Homocysteine assay kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The refe introduction homocysteine is a thiolcontaining amino acid produced by the intracellular demethylation of methionine. Dec, 2019 the detection was carried out with reference to the instructions of the corresponding kits and instruments. Homocysteine elisa kit is intended for the quantitative determination of total l homocysteine in plasma or serum. An antibody specific for hcy has been precoated onto a microplate. Abclonal provide all the timesavings, convenience and performance of a highend, commercial elisa kit for the competitive price.
Elisa and matched antibody pair kits cell and tissue imaging tools cellular and biochemical assays. All species homocysteine quant elisa kit sandwich lsbio. The kit has a detection sensitivity limit of 10 ngml homocysteine bsa. Product name human hcy homocysteine elisa kit catalogue no. Homocysteine assay kit fluorometric ab228559 allows for quantification of total homocysteine in biological fluids such as plasma and serum. Elabscience specializes in the selfdeveloped homocysteine hcy colorimetric assay kit enzyme circulation method, strict quality control, fast delivery, ensure your experiment needs. A competitive elisa kit for the quantitative measurement of homocysteine in serum, plasma, culture supernatants and other biological fluids.
The homocysteine hplc assay kit is for research use only and should not be used for diagnostic procedures. To avoid evaporation of the reagents, the reagent pack kit should not be kept open in the abbott imx analyzer when a run is finished. Rat homocysteine hcy elisa kit employs a twosite sandwich elisa to quantitate hcy in samples. Homocysteine is a chemical compound with the formula hsch2ch2chnh2co2h.
Mouse homocysteine hcy elisa kit employs a twosite sandwich elisa to quantitate hcy in samples. Standards and samples are pipetted into the wells and any hcy present is bound by the immobilized antibody. Correlation of tsh with homocysteine and lipid profile in hypothyroidism a malik, et al,j liaquat uni med health sci,2018. When stored and handled as directed, reagents are stable until the expiration date.
Biomatik elisa kits are not typically preassembled as finished products due to limited shelf life. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine selective enzyme, generating an intermediate product. To meet every single need of the customer we aim to provide high quality at best price and service with our professional staff. The maxdiscovery homocysteine enzymatic assay kit has the capacity for 48 determinations or testing of 24 samples in duplicate assuming 12 wells for standards. Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid samples. Elisa kit suppliers from china shanghai korain biotech co. General homocysteine elisa kit hcygeneral elisa kits. The homocysteine hplc assay kit includes all reagents for preparation and separation of the samples with exception of the columns ic2801rp and the controls ic2801ko. Homocystein elisa,homocystein,stroke, heart attack, homocystein elisa, homocystein. After removing any unbound substances, hrpconjugated mouse. Elisa kits and reagents for therapeutic mabs, biosimilars, and proteins.
General homocysteine elisa kit hcy rk00671 suitable for serum, plasma, cell culture supernatants and urine in general. The antibody shop, the marketplace with over a million primary and secondary antibodies, elisa kits, qpcr kits, ria kits, assay kits and reagents. This package insert must be read in its entirety before using this product. All species homocysteine elisa kit sandwich elisa user manual catalog no. For this assay, 300 ll of medium bww containing 20 9 106 cells from the high and lowdensity regions of percoll gradients were. Effect of seabuckthorn seed oil in reducing cardiovascular risk factors. Pdf diabetic retinopathy dr is the most common cause of blindness in people under the age of 65.
Homocysteine elisa kit cambridge bioscience limited. The eagle biosciences sadenosyll homocysteine sah elisa assay kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. The kit has a detection sensitivity limit of 10 ngml homocysteinebsa. Bioassay technology laboratory bt lab is a brand of shanghai korain biotech co ltd specialized in lab supplies of elisa kit, antibodies, protein for life science research. Aviva systems biology homocysteine kit human okeh02557 is based on standard sandwich enzymelinked immunosorbent assay technology.
The diazyme homocysteine enzymatic a ssay kit is intended for the in vitro quantitative determination of total l homocysteine in serum or plasma. Homocysteine hcy is a thiolcontaining amino acid produced by the intracellular demethylation of methionine. Pdf enzyme conversion immunoassay for determining total. The eagle biosciences sadenosyll homocysteine sah elisa assay kit is intended for the quantitative determination of sadenosyll homocysteine sah in serum or plasma. Human hcyhomocysteine elisa kit wuhan fine biotech co. Homocysteine assay kit fluorometric k531 biovision, inc. Elisa kit for the detection of homocysteine in the research laboratory the antibodyshop, antibodies, elisa kits, reagents. This assay is a competitive elisa where samples and an anti homocysteine antibody are added to a plate coated with a homocysteine conjugate. Description and principle the elisa genie hcy elisa kit assay can assay for the specific analyte in the. Elisa kit for the detection of homocysteine in the research. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Mouse homocysteine hcy elisa kit this product is suitable for in vitro quantitative detection of mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant hcy concentration.
Hcy homocysteine elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 4. Human alpha 1 antitrypsin elisa kit serpina1 ab108798 abcam. Elisa kits and reagents for infectious disease and vaccine research. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. After removing any unbound substances, hrpconjugated rat. Each column is can be utilized for approximately 500 samples depending on column care and sample type.
Validated in wb, ip, elisa, ihc, dot and tested in mouse, human. Total homocysteine thcy plasma abbott imx homocysteine. Total homocysteine thcy plasma abbott imx homocysteine hcy. The assay can assist in diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria.
Homocysteine elisa kit alpha diagnostic international. A longitudinal controlled trial on hypertensive subjects. It is a sandwich assay which can detect homocysteine down to 0. Minor changes may occur to the detection range, sensitivity, and. User manual competitive eia kit mouse homocysteine. Correlation of serum lipids, lipoproteins and homocysteine levels in hypothyroid patients of northwest india kuldip singh, et al,international journal of scientific research,2018.
Lsf10591 it is important that you read this entire manual carefully before starting your experiment. Despite a stay at home advisory being put in place in maryland, usa, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. It is a homologue of the naturallyoccurring amino acid cysteine, differing in that its sidechain contains an additional methylene ch2 group before the thiol sh group. Homocysteine is a thiolcontaining amino acid produced by the intracellular demethylation of methionine. Involvement of homocysteine, homocysteine thiolactone, and. Technical manual rat homocysteine hcy elisa kit catalogue.
Final absorbance was measured at 450 nm using an elisa plate reader. Homocysteine is an amino acid intermediate that is synthesized from methionine via sadenosylmethionine sam and sadenosylhomocysteine sah. Smaller amounts of reduced homocysteine and disulfide homocystine hcysshcy are present. Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid. Faulty homocysteine recycling in diabetic retinopathy. Cell biolabs homocysteine elisa kit is a competitive enzyme immunoassay developed for the detection and quantitation of homocysteine in plasma, serum, lysates, or other biological fluid samples.
Homocysteine elisa the axis homocysteine enzyme immunoassay is intended for the quantitative determination of total homocysteine in plasma or serum. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Buy hcy elisa kit, human hcy homocysteine elisa kit mbs766119 product datasheet at. The architect homocysteine reagent kit may be stored on board the architect i system for a maximum of 30. The architect homocysteine reagent kit must be stored at 28c in an upright position and may be used immediately after removal from 28c storage. Human homocysteine elisa kit allows for the in vitro quantitative determination of homocysteine concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids. Samples and biotin labeling antibody are added into elisa plate wells and washed out with pbs or tbs. Biovisions homocysteine assay kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. For additional information on system and assay technology, refer to the. Standards preparation standards corresponding to 0. If you have problems technical service contact information. The sample and conjugate compete for antibody binding, which. This assay is a competitive elisa where samples and an antihomocysteine antibody are added to a.
Our assay kits use a competitive elisa technique and each kit comes with the hcy precoated on a 96well microtiter plate. Buy homocysteine elisa kit, human homocysteine elisa kit mbs7252797 product datasheet at. The homocysteine hplc column can be used with eagle biosciences homocysteine hplc assay kit hcy31h100. Hcyhomocysteine elisa kit from elisa genie is a precoated immunoassay with a sensitivity of.
Increased severity of diabetic retinopathy was found to correlate with an increase in the serum levels of. Increased serum level of homocysteine correlates with. The loss rate of this kit is less than 10% within the expiration date under appropriate storage conditions. Gbioscienses immunotag mouse hcy homocysteine elisa kit quantity. Measurement of homocysteine level the concentration of hcy in the blood and retinas of humans and different animal models of diabetes, representing type 1 and type 2 diabetes stztreated mice, dbdb mice and stztreated ratswas determined using an hcy enzyme linked immunosorbent assay elisa kit from cell.
Human homocysteine elisa kit, human homocysteic acid elisa kit, human hcy elisa kit, human homocysteine acid elisa kit intended use this immunoassay kit allows for the in vitro quantitative determination of homocysteine, homocysteine concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids. It is a homologue of the amino acid cysteine, differing by an additional methylene bridge ch2. This immunoassay kit allows for the in vitro quantitative determination of general homocysteine concentrations in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The axis homocysteine enzyme immunoassay is intended for the. The eagle biosciences homocysteine hplc assay kit makes it possible to determine homocysteine in an easy, fast and precise method. Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease. Mouse homocysteine elisa kit competitive eia user manual catalog no. Enzymatic homocysteine 3 reagent diazyme laboratories. Eh4011 detection method competitive elisa, coated with antigen application hcy elisa kit allows for the in vitro quantitative determination of hcy concentrations in plasma, tissue homogenates and other biological fluids. Principle of the assay the microtiter plate provided in this kit has been precoated with an antibody specific to homocysteine. Sandwich kit the sample with an unknown amount of antigen is immobilized on a precoated plate either nonspecifically via adsorption to the surface or specifically via capture by another antibody specific to the same antigen. Gbioscienses immunotag mouse hcy homocysteine elisa kit.
Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum. This elisa kit is used for quantitative measurement of homocysteine in serum, plasma, tissue homogenates and other biological fluids. Statistical analysis was used to assess the correlations between the study variables. Homocysteine hplc assay column eagle bio hplc assay column. The shelf life is 12 months when the kit is properly stored. Maxdiscovery homocysteine enzymatic assay kit specs. Human homocysteine elisa kit can be provided from creative diagnostics. The homocysteine hplc assay kit is for the quantitative determination of homocysteine in plasma and serum and. The elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Sta670, san diego, ca, usa, according to the protocol provided with the kit. The eagle biosciences homocysteine hplc assay kit is intended for the quantitative determination of homocysteine in plasma and serum. Csbe814h for the quantitative determination of endogenic human homocysteine hcy concentrations in serum, plasma, urine, tissue homogenates.
The stability of the mouse homocysteine elisa kit is determined by the loss rate of activity. Levels of homocysteine were measured in the retinal homogenate 15. For the quantitative determination of endogenic human homocysteine hcy. Send me a copy of this email i agree to the terms and conditions. Competitive kit in a competitive elisa assay, sample antigen and labeled antigen compete for capture antibody binding. Homocysteine elisa kit lsf10591 is a 96well enzymelinked immunosorbent assay for the quantitative detection of all species homocysteine in plasma, serum. Rat homocysteine assay kit fluorescence mouse homocysteine assay kit. This kit is not approved for use in humans or for clinical diagnosis. Maxdiscovery homocysteine enzymatic assay kit bioo scientific. Homocysteine elisa kit okeh02557 quantitative competitive. Hcy is exported into plasma where it circulates mostly in its oxidized forms bound to plasma proteins. K531100 homocysteine assay kit fluorometric biovision.
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